Detection of Cholecystokinin-B/Gastrin Receptor mRNA Expressing Cells in the Stomach of Hypergastrinemic Rats

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Abstract

Enterochromaffin-like (ECL) cells, which are numerous in the oxyntic mucosa of the rat stomach, proliferate in the presence of hypergastrinemia. We investigated the distribution of cells expressing gastrin receptor (GR) in Sprague-Dawley rats in which hypergastrinemia was induced by partial gastric corpectomy. The ECL cell density was determined by immunohistochemical staining for histamine. The expression of GR mRNA was investigated using digoxigenin-labeled complementary RNA probes. The labeling index of the generative cell zone was determined by <sup>3</sup>H-thymidine autoradiography. The ECL cell density and the labeling index in the generative cell zone increased in association with increased plasma levels of gastrin after 24 weeks (p<0.05). The expression of GR mRNA was seen not only in ECL, generative, parietal and chief cells, but also in stromal cells of hypergastrinemic rats. The expression of GR mRNA became more marked in the oxyntic mucosa as plasma levels of gastrin increased, and it was suggested that GR mRNA expression acts as one indication in the development of ECL cell hyperplasia.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 28(4), 323-331, 1995-08-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  46

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008604114
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00445991
  • Data Source
    CJP  CJPref  J-STAGE 
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