Distribution of the Extracellular Free Ca^<2+> in the Periodontal Ligament and Periosteum during Experimental Tooth Movement Using Fluorescent Free Ca^<2+> Indicator "Fluo-3"
Distribution of extracellular free Ca<sup>2+</sup> was examined in the rat periodontal ligament and periosteum of alveolar bone during tooth movement, using fluo-3. To stimulate bone formation and osteoclastic bone resorption, upper molars of adult rats were moved lingually for 7 days. After careful removal of the chestal ribs, fluo-3 was injected into the left ventricle of throbbing hearts, immediately after clamping the descending aortae with forceps. 90sec after the injection, the upper jaw was excised out rapidly and immersed in liquid nitrogen. 5μm unfixed undecalcified frozen sections from the frozen tissues were prepared for fluorescent microscopy and also electron microprobe analysis (EPMA). Green fluorescence was emitted from the whole periodontal space and pulp cavity. The pattern of bone formation in the periodontal ligament was quite different from that in the periosteum. A considerable amount of osteoid formed in the buccal periodontal ligament (tension side) and also in the periosteum of lingual alveolar bone (compensative bone apposition). The fluorescence emitted from the osteoid was more intense than that from the periodontal ligament or subperiosteal tissue. EPMA data also showed a higher peak of Ca in the osteoid than in the periodontal ligament or subperiosteal tissues. In addition, a narrow linear green fluorescence was emitted from the interface between the osteoclasts and the bone in the lingual periodontal ligament (pressure side). These results suggest that osteoid contains more free Ca<sup>2+</sup> than periodontal ligament and subperiosteal tissue.
- Acta histochemica et cytochemica
Acta histochemica et cytochemica 28(4), 381-387, 1995-08-01
JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY