Two Monoclonal Antibodies Distinguish Growth Cartilage from Other Types of Cartilage and Other Tissues
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Two monoclonal antibodies (mAbs) were raised as probes to study the specificity of a growth cartilage (GC). Immunized antigen was a homogenate of cultured GC cells from young rabbit ribs. Raised mAbs were investigated as to their reactivity with undecalcified or decalcified paraffin-embedded rabbit tissues. By immunohistochemical examination of the young rabbit tissues, one mAb, mAb-A, reacted with non-osteogenic cartilage, muscle, bone marrow cells, dermis, epidermis, gut, tendon, lung, liver, heart, spleen, and thymus, but not with the growth cartilage. The other mAb, mAb-B, however, reacted specifically with late hypertrophic and calcified growth cartilage zones, but not with other non-osteogenic cartilage or other tissues.<br>These two mAbs are useful probes to distinguish osteogenic cartilage from nonosteogenic cartilage and other tissues by negative reactivity as in mAb-A, and by positive reactivity as in mAb-B. Further-more, these results imply the existence of certain molecules to distinguish osteogenic cartilage from non-osteogenic cartilage.
- ACTA HISTOCHEMICA ET CYTOCHEMICA
ACTA HISTOCHEMICA ET CYTOCHEMICA 28(5), 417-422, 1995-10-01
JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY