Two Monoclonal Antibodies Distinguish Growth Cartilage from Other Types of Cartilage and Other Tissues.

• Okihana Hiroyuki

  Department of Orthopedic Surgery, National Defense Medical College

抄録

Two monoclonal antibodies (mAbs) were raised as probes to study the specificity of a growth cartilage (GC). Immunized antigen was a homogenate of cultured GC cells from young rabbit ribs. Raised mAbs were investigated as to their reactivity with undecalcified or decalcified paraffin-embedded rabbit tissues. By immunohistochemical examination of the young rabbit tissues, one mAb, mAb-A, reacted with non-osteogenic cartilage, muscle, bone marrow cells, dermis, epidermis, gut, tendon, lung, liver, heart, spleen, and thymus, but not with the growth cartilage. The other mAb, mAb-B, however, reacted specifically with late hypertrophic and calcified growth cartilage zones, but not with other non-osteogenic cartilage or other tissues.<br>These two mAbs are useful probes to distinguish osteogenic cartilage from nonosteogenic cartilage and other tissues by negative reactivity as in mAb-A, and by positive reactivity as in mAb-B. Further-more, these results imply the existence of certain molecules to distinguish osteogenic cartilage from non-osteogenic cartilage.

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 28 (5), 417-422, 1995

  日本組織細胞化学会

キーワード

• Monoclonal antibody
• Growth cartilage
• Immunohistochemistry
• Osteogenic cartilage

詳細情報

• CRID: 1390282679839426176
• NII論文ID: 10008604468
• NII書誌ID: AA00508022
• DOI: 10.1267/ahc.28.417
• ISSN: 13475800
• ISSN: 00445991
• Web Site: http://www.jstage.jst.go.jp/article/ahc1968/28/5/28_5_417/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1996172658
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可