Matrix Cells Dissociated from the Mouse Forebrain Vesicle Reaggregate to Form Neural Tube-like Organoid Structures in vitro

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Author(s)

    • FUSHIKI Shinji
    • Department of Dynamic Pathology, Research Institute for Neurological Diseases and Geriatrics, Kyoto Prefectural University of Medicine
    • FUJITA Setsuya
    • Department of Dynamic Pathology, Research Institute for Neurological Diseases and Geriatrics, Kyoto Prefectural University of Medicine

Abstract

An <i>in vitro</i> study employing dissociated matrix cells obtained from the rostral part of mouse neural tube or forebrain vesicle was done in an attempt to elucidate their cellular characteristics. We found a reaggregate formation with an organoid architecture. The organization of the reaggregate was studied by transmission as well as scanning electron microscopy, and by immunocytochemistry. After three days in a stationary culture, dissociated matrix cells formed a spherical, organoid reaggregate having a central lumen. The size of the reaggregate varied from approximately 100μm to 200μm. The transmission electron microscopy revealed that the reaggregate wall is composed of two types of cells; one has an electron-lucent cytoplasm with few organelles except for polysomes, whereas the other contains an electron-dense cytoplasm with many microtubules and mitochondria. The latter type of cell was located in the marginal portion of the wall intermingled with a large number of cellular processes. These cells were immunostained with anti-neurofilament antibodies and regarded as neuroblasts. The luminal surface was composed predominantly of the former cell type showing well-developed junctional complexes in between them and having cilia on its luminal side. Mitotic figures were frequently demonstrable adjacent to the lumen.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 28(5), 453-459, 1995-10-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  24

Codes

  • NII Article ID (NAID)
    10008604604
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00445991
  • Data Source
    CJP  J-STAGE 
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