Spatiotemporal Immunolocalization of Stage-Spacific Embryonic Antigen-1 and Related Antigens in the Developing Trachea and Lung of Fetal Hamsters

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Author(s)

    • ITO Takaaki
    • Department of Pathology, Yokohama City University School of Medicine
    • UDAKA Naoko
    • Department of Pathology, Yokohama City University School of Medicine

Abstract

We examined the immunolocalization of stage-specific embryonic antigen-1 (SSEA-1) and related antigens in the developing trachea and lungs of fetal hamsters both <I>in vitro</I> and <I>in vitro</I>. On gestational days 10-16 (the day ot birth), fetal tracheas and lungs were fixed in phosphate-buffered paraform-aldehyde and frozen. Frozen sections were incubated with monoclonal antibodies against SSEA-1 (Le<SUP>x</SUP> hapten), sialyl SSEA-1 carrying i antigen and fucosyl SSEA-1 (Le<SUP>Y</SUP>hapten). Some sections were treated with FITC-labeled secondary antibody for observation by confocal laser microscopy, and others were treated with colloidal gold-labeled secondary antibody for ultrastructural observation. In the developing tracheas, preferential staining in the ventral and lateral tracheal epithelium appeared for all of the antibodies by day 15, and ultrastructural study demonstrated that the regional difference in staining occurred regardless of cell type. In the developing lungs, positive sialyl SSEA-1-i antigen immunostaining was seen in the terminal portion of the epithelium by day 14, and the staining disappeared during the postnatal period. An <I>in vitro</I> study using explant organ culture of gestational day-11 lung with trachea revealed similar immuno-localization patterns. In the cultured trachea, the regional difference in immuno-staining was also seen with development of cartilage and smooth muscle, and in the cultured lung, the staining intensity became stronger in the terminal epithelium than in larger airway epithelium.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 28(6), 513-521, 1995-12-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  32

Codes

  • NII Article ID (NAID)
    10008604695
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00445991
  • Data Source
    CJP  J-STAGE 
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