Regeneration of Paneth Cells after Dithizone Treatment

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Author(s)

    • MIYAKE Naoki
    • Department of Pathology and Biology of Diseases, Kyoto University Graduate School of Medicine
    • SAWADA Mitsutaka
    • Department of Pathology and Biology of Diseases, Kyoto University Graduate School of Medicine
    • HIAI Hiroshi
    • Department of Pathology and Biology of Diseases, Kyoto University Graduate School of Medicine

Abstract

Injection of dithizone, a zinc chelating agent, induced selective killing of intestinal Paneth cells within a few min by forming toxic insoluble zinc-dithizonate complexes. However, regeneration of Paneth cells took place right after dithizone treatment and resumed the normal level as early as 72 hr with over-shooting persistent for 2 weeks. The process or regeneration in intestinal crypts was studied by means of morphology, uptake of the thymidine analog 5-bromo-2′-deoxy-uridine and immunohistochemical staining of Paneth cell differentiation markers such as lysozyme and zinc-binding proteins. It was found that Paneth cells regenerated without cell division and DNA synthesis apparently by direct transformation of non-Paneth crypt base columnar cells. Dithizone induced a transient wave of cell growth in all compartments of the intestinal crypt.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 28(6), 549-553, 1995-12-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  13

Codes

  • NII Article ID (NAID)
    10008604818
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00445991
  • Data Source
    CJP  J-STAGE 
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