A New Approach to Study the Relationship between Calcium Waves and Diastolic [Ca^<2+>]_i in Heart Muscle Cells

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Author(s)

    • TAKAHASHI Akiyuki
    • Department of Pathology and Cell Regulation, Kyoto Prefectural University of Medicine
    • TAKAMATSU Tetsuro
    • Department of Pathology and Cell Regulation, Kyoto Prefectural University of Medicine

Abstract

In the present study, changes in calcium waves with alternation of diastolic [Ca<SUP>2+</SUP>] <SUB>i</SUB> were investigated in a single common rat neonatal cultured heart muscle cell without changes in extracellular conditions. Heart muscle cells were loaded with fluo-3 by the “whole cell clamp” technique. Rapid chan ges in diastolic [Ca<SUP>2+</SUP>] <SUB>i</SUB> by variations in the membrane potential clarified the influences on calcium waves. When the membrane potential was held at 0 mV, inter wave basal [Ca<SUP>2+</SUP>] <SUB>i</SUB> were maintained at a high level. The membrane-holding potential was then momentarily altered to -80 mV and the basal [Ca<SUP>2+</SUP>] <SUB>i</SUB> registered a lower level within 1 sec. During these events, spontaneous calcium waves were analyzed by confocal laser scanning microscopy (X-t scanning mode). The frequency and velocity of calcium waves at 0 mV significantly increased compared with those at-80 mV. Moreover, these changes were more pronounced with increasing extracellular [Ca<SUP>2+</SUP>]. These results suggest that diastolic [Ca<SUP>2+</SUP>] <SUB>i</SUB>; directly influences calcium waves.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 28(6), 565-568, 1995-12-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  22

Cited by:  2

Codes

  • NII Article ID (NAID)
    10008604853
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00445991
  • Data Source
    CJP  CJPref  J-STAGE 
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