Simultaneous Demonstration of Microscopic Anatomy of Brainstem, Nissl Substance, Lipofuscin and Cytological Changes Using a Combination Method

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Author(s)

    • PATRO Isan K.
    • Neurobiology and Ageing Unit, School of Studies in Zoology, Jiwaji University
    • PATRO Nisha
    • Neurobiology and Ageing Unit, School of Studies in Zoology, Jiwaji University
    • SINGHAL Praveena
    • Neurobiology and Ageing Unit, School of Studies in Zoology, Jiwaji University

Abstract

The proposed combination of three histochemical methods facilitates the demonstration of microscopic anatomy, Nissl content of neurons, lipofuscin accumulation and associated cytopathological changes, if any, in one brain section. Form-aldehyde fixed paraffin sections were stained with a modified Weil's method, cresyl violet and pyronin Y in sequence, dehydrated, cleared and mounted in DPX. The slides were studied under a fluorescence microscope. The fibre system stained grey. The Nissl substance and lipofuscin stained violet and pink, respectively. On exposure to UV light, the pink stained pigment emitted golden-yellow fluorescence, resembling the auto-fluorescence of unstained lipofuscin. The final results were remarkably reproducible.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 29(3), 237-241, 1996-06-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  18

Codes

  • NII Article ID (NAID)
    10008605564
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00445991
  • Data Source
    CJP  J-STAGE 
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