DNA Synthesis in the Tracheae of Aging Mice by Light and Electron Microscopic Radioautography

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Author(s)

    • SUN Lin
    • Department of Anatomy and Cell Biology, Shinshu University School of Medicine
    • GAO Fulu
    • Department of Anatomy and Cell Biology, Shinshu University School of Medicine
    • JIN Chunji
    • Department of Anatomy and Cell Biology, Shinshu University School of Medicine
    • NAGATA Tetsuji
    • Department of Anatomy and Cell Biology, Shinshu University School of Medicine

Abstract

The present study was undertaken to elucidate the age-related changes of DNA synthesis and morphology of tracheal cells in aging mice by light and electron microscopic radioautography. The tracheae of 8 groups of mice from fetal day 18 to 2 years after birth were labeled with <SUP>3</SUP>H-thymidine <I>in vitro</I>. The results demonstrated that the DNA syntheses and morphology of tracheal cells in aging mice changed due to aging. The radioautographic examinations revealed that the DNA syntheses of the nonciliated and basal cells reached their maxima on fetal day 18, then fell from postnatal 3 das onwards. The activity of DNA synthesis of ciliated cell was very low in the fetal stage. Ciliated cells could not synthesize DNA and proliferate in the postnatal stage, which were derived by the division and transformation of basal cells. On the other hand, the DNA synthesis of chondrocytes was the highest on embryonic day 18, and rapidly declined on postnatal day 3. The chondrocytes lost the ability of synthesizing DNA at 2 months after birth. The DNA syntheses of other cells (including fibroblasts, smooth muscle and glandular cells) were the highest on fetal day 18 and fell markedly on the third day after birth. They increased again at 1 week, then decreased progressively due to aging.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 30(2), 211-220, 1997-04-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  52

Cited by:  2

Codes

  • NII Article ID (NAID)
    10008606658
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00445991
  • Data Source
    CJP  CJPref  J-STAGE 
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