Lowering Extracellular pH Raises Intracellular Calcium in Cultured Rat Astrocytes

Access this Article

Search this Article

Author(s)

    • NAGAOKA Takanori
    • Department of Pathology and Cell Regulation, Kyoto Prefectural University of Medicine
    • MASUDA Atsushi
    • Department of Pathology and Cell Regulation, Kyoto Prefectural University of Medicine
    • TAKAMATSU Tetsuro
    • Department of Pathology and Cell Regulation, Kyoto Prefectural University of Medicine

Abstract

The relationship between extracellular pH and intracellular calcium concentration ([Ca<SUP>2+</SUP>] <SUB>1</SUB>) in primary culture of neonatal rat astrocytes with Ca<SUP>2+</SUP>-indicator dye indo-1/acetoxymethyl ester and a confocal microscope was investigated. In 65 of 86 astrocytes studied, lowering extracellular pH by changing the perfusate solution from pH 7.4 to 6.4 was followed by increases in [Ca<SUP>2+</SUP>] <SUB>1</SUB> in cytoplasm. The increases in [Ca<SUP>2+</SUP>] <SUB>1</SUB> were seen on stimulation and/or during reperfusion. Of all the cells, 45.4% showed a transient pattern and 30.2% manifested a sustained pattern. The results suggest that a short interval but excessive acidic stimulation may be one of the signals that raises [Ca<SUP>2+</SUP>] <SUB>1</SUB> in a certain population of cerebral astrocytes.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 30(2), 221-225, 1997-04-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  34

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008606711
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00445991
  • Data Source
    CJP  CJPref  J-STAGE 
Page Top