Ki-67 Antigen Retrieval in Formalin- or Ethanol-fixed, Paraffin-embedded Tissues : An Enhancement Method for Immunohistochemical Staining with Autoclave Treatment

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Author(s)

    • TSUJI Yoshiro
    • Department of Orthopaedic Surgery, Kyoto Prefectural University of Medicine
    • HIRASAWA Yasusuke
    • Department of Orthopaedic Surgery, Kyoto Prefectural University of Medicine
    • SERRA Massimo
    • Department of Research Oncology, Rizzoli Orthopaedic Institute

Abstract

We developed a new immunohistochemical method to visualize Ki-67 antigen in formalin-fixed, paraffin-embedded sections of bone tumors. The method uses autoclave pretreatment of sections immersed in citrate buffer before incubation with the MIB 1 antibody, which reacts with the Ki-67 gene product. MIB 1 antibody showed strong and discrete staining of cell nuclei in proliferating tumor cells. This method also retrieved antigen in paraffin-embedded sections of alcohol-fixed tissue. Therefore, tissues previously considered unsuitable for immunohistochemical analysis can be studied after autoclave treatment.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 30(3), 251-255, 1997-06-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  8

Cited by:  2

Codes

  • NII Article ID (NAID)
    10008606822
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00445991
  • Data Source
    CJP  CJPref  J-STAGE 
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