Long-Term Organotypic Slice Culture of the Neonatal Mouse Liver

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Author(s)

    • HARADA Yoshinori
    • Third Department of Internal Medicine and Kyoto Prefectural University of Medicine
    • IWAI Masaki
    • Third Department of Internal Medicine and Kyoto Prefectural University of Medicine
    • MORI Takahiro
    • Third Department of Internal Medicine and Kyoto Prefectural University of Medicine
    • TADA Kazunobu
    • Third Department of Internal Medicine and Kyoto Prefectural University of Medicine
    • OKANOUE Takeshi
    • Third Department of Internal Medicine and Kyoto Prefectural University of Medicine
    • KOBORI Nobuhide
    • Department of Dynamic Pathology and Research Institute for Neurological Diseases & Geriatrics, Kyoto Prefectural University of Medicine
    • FUSHIKI Shinji
    • Department of Dynamic Pathology and Research Institute for Neurological Diseases & Geriatrics, Kyoto Prefectural University of Medicine
    • KASHIMA Kei
    • Third Department of Internal Medicine and Kyoto Prefectural University of Medicine

Abstract

We cultured 3-day-old mouse livers for organotypic slice culture using a roller-tube technique to maintain parenchymal cells for a long term. The sliced tissues in medium without growth factors gradually spread and flattened for 3 weeks. Parenchymal cell formed a trabecular structure and albumin-immunoreactivity was seen in them in light and ultrastructural immunoperoxidase studies. Cholangiolar cells formed glandular structures which had microvilli on the inner facing membrane and basement membrane on the basal area. There were also well-developed tight junctions and few cytoplasmic organella. These findings suggest that our organotypic slice culture of neonatal livers developed with the roller-tube technique could preserve hepatocytes and cholangiolar cells for at least 3 weeks. This culture system may be a useful tool not only to maintain parenchymal cells but also to study structural organization of the liver in ontogenesis.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 30(4), 395-399, 1997-08-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  13

Cited by:  2

Codes

  • NII Article ID (NAID)
    10008607226
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00445991
  • Data Source
    CJP  CJPref  J-STAGE 
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