Long-Term Organotypic Slice Culture of the Neonatal Mouse Liver.

DOI Web Site 被引用文献2件 参考文献13件
  • Harada Yoshinori
    Third Department of Internal Medicine, Kyoto Prefectural University of Medicine
  • Iwai Masaki
    Third Department of Internal Medicine, Kyoto Prefectural University of Medicine
  • Mori Takahiro
    Third Department of Internal Medicine, Kyoto Prefectural University of Medicine
  • Tada Kazunobu
    Third Department of Internal Medicine, Kyoto Prefectural University of Medicine
  • Okanoue Takeshi
    Third Department of Internal Medicine, Kyoto Prefectural University of Medicine
  • Kobori Nobuhide
    Department of Dynamic Pathology and Research Institute for Neurological Diseases & Geriatrics, Kyoto Prefectural University of Medicine
  • Fushiki Shinji
    Department of Dynamic Pathology and Research Institute for Neurological Diseases & Geriatrics, Kyoto Prefectural University of Medicine
  • Kashima Kei
    Third Department of Internal Medicine, Kyoto Prefectural University of Medicine

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We cultured 3-day-old mouse livers for organotypic slice culture using a roller-tube technique to maintain parenchymal cells for a long term. The sliced tissues in medium without growth factors gradually spread and flattened for 3 weeks. Parenchymal cell formed a trabecular structure and albumin-immunoreactivity was seen in them in light and ultrastructural immunoperoxidase studies. Cholangiolar cells formed glandular structures which had microvilli on the inner facing membrane and basement membrane on the basal area. There were also well-developed tight junctions and few cytoplasmic organella. These findings suggest that our organotypic slice culture of neonatal livers developed with the roller-tube technique could preserve hepatocytes and cholangiolar cells for at least 3 weeks. This culture system may be a useful tool not only to maintain parenchymal cells but also to study structural organization of the liver in ontogenesis.

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