The Intracellular Calcium Store Function of Platelet Granules

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Author(s)

    • LING Yiping
    • Department of Biophysics, Shanghai Medical University
    • DING Jian
    • Department of Biophysics, Shanghai Medical University
    • YU Zhang
    • Department of Biophysics, Shanghai Medical University
    • OGAWA Kazuo
    • Department of Anatomy Faculty of Medicine, University of Kyoto

Abstract

The atrial specific granule has been described as an intracellular Ca store (ICaS). It is reasonably proposed that other polypeptidesecretory granules could also play the function of ICaS. In the present work, high Ca concentration in the dense granule (dG) and α granule (αG) of platelets was confirmed by using x-ray microanalysis in both ultrathin cryosections and ethanolic phosphotungstic acid staining sections, and Ca<SUP>2+</SUP>-ATPase activities were found on the membrane of both the granules. Furthermore, when platelets were activated by A 23187 1 mM, thrombin 2 U/ml or ADP 200μM, both the Ca content and number of the granules decreased, which means that the Ca<SUP>2+</SUP> was released and secretion of the granules increased. It is suggested that the release Ca<SUP>2+</SUP> raise the cytosolic Ca2+ level and, thus, the secretion of the granules is promoted. In addition, it was described that the ultrastructure of the platelet granules in ultrathin cryosections was somewhat different from that in routine sections.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 30(5), 439-444, 1997-10-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  15

Codes

  • NII Article ID (NAID)
    10008607400
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00445991
  • Data Source
    CJP  J-STAGE 
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