Histochemical and Cytochemical Studies on Newt Lung Mast Cells.

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  • Hirata Junko
    Department of Biology, Tsurumi University, School of Dental Medicine
  • Nishikawa Sumio
    Department of Biology, Tsurumi University, School of Dental Medicine
  • Sasaki Fumie
    Department of Biology, Tsurumi University, School of Dental Medicine

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Mast cells which show metachromasia with toluidine blue were observed in newt (Cynops pyrrhogaster) lung connective tissue. The mast cells showed strange granules in their cytoplasm. In this study, we examined the characteristics of the mast cells by using histochemical and cytochemical methods.<BR>The mast cells were positive for PAS reaction and Alcian blue (pH 1.0). Electron microscopy showed that the mast cells stretched their cytoplasm into the collagen layer. The cytoplasm was filled with various types of granules, some homogeneous throughout, others heterogeneous, and were classified as follows: high and low electron density amorphous structure, straight lamella structure, and rough particle structure. These granules were irregular in size. They were stained by alkaline bismuth and dialyzed iron. The rough particle structure granules in particular showed stronger staining than others.<BR>In order to determine which sulfated polysaccharide was contained in the granules, we performed enzyme treatment using two enzymes chondroitinase ABC (pH 8.0) and heparinase. Our results indicated that the granules contained heparin.

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