Expression of Cystatin C in Human Histiocytic Lymphoma, U-937, Cells

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Author(s)

    • BARKA Tibor
    • Department of Cell Biology and Anatomy, Mount Sinai School of Medicine of The City University of New York
    • NOEN Hendrika van der
    • Department of Cell Biology and Anatomy, Mount Sinai School of Medicine of The City University of New York

Abstract

The established human histiocytic lymphoma U-937 cell line is of monocytic origin. U-937 cells can be induced by tumor promoters, Vitamin D<SUB>3</SUB> and retinoic acid to differentiate into macrophage-like cells. U-937 cells express the gene for the cysteine proteinase inhibitor cystatin C. This was established by Northern blot hybridizations, reverse transcriptase-polymerase chain reaction and Western blots of partially purified cystatins from extracts of U-937 cells. Induction of macrophage-like differentiation by the tumor promoters phorbol-12-myristate-13-acetate or 12-O-tetradecanoylphorbol-13-acetate, or with Vitamin D<SUB>3</SUB>, or retinoic acid reduced the steady-state level of cystatin C mRNA in U-937 cells, suggesting that the expression of the cystatin C gene varies with the differentiation state. U-937 cells secrete cystatin C. This secretion was not affected significantly by the calcium ionophore A23187, PMA, or dibutyryl-adenosine 3′: 5′-cyclic monophosphate. Since macrophages play pivotal roles in immune and inflammatory reactions involving intra- and extra-cellular proteolysis, cystatin C produced and secreted by these cells probably has a modulatory function on the action of cysteine proteinases produced or released locally.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 30(5), 483-489, 1997-10-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  29

Codes

  • NII Article ID (NAID)
    10008607609
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00445991
  • Data Source
    CJP  J-STAGE 
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