Regional Distribution of β1-4 Galactosyltransferase in Rat Epididymis

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Abstract

Membrane-bound forms of β1-4 galactosyltransferase (GalTase) are distributed <I>in trans</I> Golgi cisternae and plasma membrane, while a soluble form of the enzyme also exists in extracellular fluid. In epididymis, the soluble form has been found in luminal fluid and suggested to play a role on sperm maturation. In the present paper, regional distributions of the membrane-bound and soluble forms of GalTase were studied in rat epididymis. Immunohistochemical studies showed that a very high concentration of GalTase was localized in Golgi apparatuses of epididymal duct epithelium from initial segment to intermediate caput, although much lower amounts of GalTase were in efferent ducts, distal caput, corpus, and cauda. The same distribution pattern was also shown by biochemical assays of the microsomal GalTase activity. On the other hand, assays of the soluble GalTase activity in epididymal fluids revealed a relatively uniform distribution pattern. Even in initial segment and caput epididymis, only low levels of the activity were detected in the fluid.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 30(5), 491-495, 1997-10-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  16

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008607639
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00445991
  • Data Source
    CJP  CJPref  J-STAGE 
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