Arginine Administration Reduces Hydrogen Peroxide in Ischemia-reperfusion Endothelium of Rats

Access this Article

Search this Article

Author(s)

    • SUN Anyang
    • Department of Neurobiology, Shanghai Medical University
    • GU Yudong
    • Department of Handsurgery, Shanghai Medical University

Abstract

L-Arg as a precursor of nitric oxide (NO) can be used in experiments to observe the action of NO. The present work is aimed at exploring the effect of oral administration of L-Arg on the ischemia-reperfusion (IR) damage of endothelial cells (EC) and the clearance of reaction oxygen species in the IR carotid artery. Cerium-marked cytochemical technique was used to localize the H<SUB>2</SUB>O<SUB>2</SUB> product in endothelium. The area of Ce-H<SUB>2</SUB>O<SUB>2</SUB> denseprecipitate on the lumen surface of damaged endothelium per length of artery wall (nm2/nm) was measured. It was found that the dense precipitate was decreased to 102.1±27.6 in rats drinking tap water containing 2.5% L-arginine for 3 days, much less than in control rats drinking tap water only (203.7±31.8, p<0.01). Furthermore, the IR damage of EC was relieved markedly. Drinking tap water containing D-Arg or L-Lys had no effects either on the amount of dense precipitate or on the EC damage. N<SUP>G</SUP>-nitro- L-arginine methyl ester, an inhibitor of NO synthase, decreased the effect of L-Arg markedly. These results indicate that L-Arg administration can reduce H<SUB>2</SUB>O<SUB>2</SUB> production in the IR artery, and thus IR damage of EC is alleviated.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 30(5), 525-529, 1997-10-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  16

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008607765
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00445991
  • Data Source
    CJP  CJPref  J-STAGE 
Page Top