Tetramethylbenzidine Polyvinylpyrrolidone Platinum Reaction for Detection of Tracer Horseradish Peroxidase

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Author(s)

Abstract

3, 3′, 5, 5′-tetramethylbenzidine polyvinylpyr-rolidone platinum (TMB-PVP-PT) reaction was moderately sensitive to horseradish peroxidase (HRP), and gave an improved image of the tracer localization. When HRP-injected mouse liver and submandibular gland specimens were treated with this reagent, a blue reaction product in fine granular shape was formed only at the sites where the tracer was localized, while no endogenous peroxidase activity was detected at all. Some attempt to enhance the sensitivity of this reagent to peroxidase was also made. This reaction was suitable for making permanent microscopic preparations.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 30(5), 545-550, 1997-10-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  21

Codes

  • NII Article ID (NAID)
    10008607841
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00445991
  • Data Source
    CJP  J-STAGE 
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