Qualitative and Quantitative Analysis of Various Classes of Alcohol Dehydrogenase in Structures of Rat Skin

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Abstract

The three classes of alcohol dehydrogenase (ADH) in the rat which have been described at the protein level were subjected to qualitative and quantitative analysis by histochemical and microelectrophoretic means. Enzyme activity was studied in the epidermis, sebaceous glands, hair roots, stratum papillare, stratum reticulare and panniculus carnosus of the rat skin. Total ADH activity was histochemically demonstrable in all these structures. After electrophoretic separation of microdissected samples by a newly developed ultrathin-layer gel electro phoresis technique, no class I ADH activity was found in any of the above-mentioned structures, whereas class III ADH activity was ubiquitously detectable, although only at a faint level. Class IV ADH however, showed high activity in the epidermis and the sebaceous glands. Maximum activity could be attributed to the fibroblasts of the stratum papillare. The metabolic role of class IV ADH in the skin is discussed with respect to retinoic acid synthesis and the degradation of lipid peroxidation products.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 30(5), 567-574, 1997-10-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  33

Codes

  • NII Article ID (NAID)
    10008607918
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00445991
  • Data Source
    CJP  J-STAGE 
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