Method for Measuring Gap Junctional Intercellular Communication by Fluorescence Recovery after Photobleaching

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Author(s)

    • LUO Shenqiu
    • Department of Cell Biology and Medical Genetics, First Military Medical University
    • MO Yongyan
    • Department of Cell Biology and Medical Genetics, First Military Medical University

Abstract

The present study was designed to establish a method for measuring cultured neuroglial gap junctional intercellular communication (GJIC) of rat cerebral cortices by fluorescence recovery after photobleaching (FRAP) analysis technique. As a result of the managements, such as cell culture, fluorescence staining, fluorescence excitement, laser scanning and computer analysis <I>et al.</I>, fluorescence inside all selected cells recovered in different degrees, i. e., fluorescence relative intensities in the 1st-5th cell raised 14%, 28%, 43%, 17.5% and 12.5% respectively after scanning 15.4 min, the mean value of recovery was 18.57±10.06% and the mean rate of fluorescence recovery was 1.223±0.785%/min for three samples (n=14 cells). The result showed that the FRAP is an ideal method for measuring GJIC.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 30(5), 575-578, 1997-10-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  16

Codes

  • NII Article ID (NAID)
    10008607952
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00445991
  • Data Source
    CJP  J-STAGE 
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