Immunohistochemistry of Intercellular Junctinos in Developing Ciliary Body of the Rabbit Eye
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The expression of intercellular junctions in developing ciliary epithelium of the rabbit eye was studied utilizing monoclonal antibodies to two major proteins comprising these junctions, connexin43, for gap or communicating junctions, and ZO-1, for tight or occluding junctions. The immunoreactivity of cx43 appears early, before distinct recognition of the differentiating ciliary body. The appearance of immunoreactivity to ZO-1 was strongly positive only in the later phases of gestation. These findings imply 1) that the gap junctions tend to solicit a cooperativity between the two cell layers of the ciliary epithelium during early development ensuring communication between the outer (blood side) and inner (eye side) two layers of this uniquely constructed ciliary epithelium, and 2) that the sequential development of the tight junctions to create a secretory gradient occurs when the eye no longer receives its fetal internal vascular supply and now requires a nourishing aqueous humor.
- ACTA HISTOCHEMICA ET CYTOCHEMICA
ACTA HISTOCHEMICA ET CYTOCHEMICA 30(5), 579-592, 1997-10-01
JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY