HVEM Observation of Phagosome-Lysosome Fusion in the Pigment Epithelium.

• Saito Takuma

  Department of Anatomy, Jichi Medical School

• Takizawa Toshihiro

  Department of Anatomy, Jichi Medical School

• Yashiro Takashi

  Department of Anatomy, Jichi Medical School

• Akahoshi Takayuki

  Department of Ophthalmology, The Mitsui Memorial Hospital

抄録

The fusion of lysosomes to phagosomes was observed under high voltage electron microscopy, in 4μm thick rat retinal sections with the aid of acid phosphatase cytochemistry. The study of thick sections facilitates the observation of the moment of fusion in stereo view from two tilted pictures. From this study, the contents of the lysosome pored into the phagosome through the orifice, shortly after the collision of the two organelles. The hydrolytic enzymes such as acid phosphatase spread in a sheet under the limiting membrane of the phagosome to finally form a balloon of the reaction product. In some case the ballooning appeared to be doubled. The outer skin of the reaction product may be the result of a wrapping mechanism of phagolysosomes.

収録刊行物

• Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ

  Ａｃｔａ　Ｈｉｓｔｏｃｈｅｍｉｃａ　ｅｔ　Ｃｙｔｏｃｈｅｍｉｃａ 30 (5/6), 675-683, 1997

  日本組織細胞化学会

キーワード

• High voltage electron microscopy
• Enzyme histochemistry
• Acid phosphatase
• Retinal pigment epithelium
• Phagosome-lysosome fusion

詳細情報

• CRID: 1390282679840071680
• NII論文ID: 10008608227
• NII書誌ID: AA00508022
• DOI: 10.1267/ahc.30.675
• ISSN: 13475800
• ISSN: 00445991
• Web Site: http://www.jstage.jst.go.jp/article/ahc1968/30/5-6/30_5-6_675/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1998174994
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可