Modulation of Osteonectin and Osteopontin Expression during Fracture Healing of Mouse Bone Tissue

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Author(s)

    • KITAZAWA Riko
    • Second Department of Pathology, Kobe University School of Medicine
    • MAEDA Sakan
    • Second Department of Pathology, Kobe University School of Medicine
    • MIZUNO Kosaku
    • Department of Orthopaedic Surgery, Kobe University School of Medicine
    • KITAZAWA Sohei
    • Second Department of Pathology, Kobe University School of Medicine

Abstract

We investigated the expression of osteonectin and osteopontin during fracture healing of mouse tibiae by <i>in situ</i> hybridization (ISH) and the effects of fibrin(ogen) on the expression of bone matrix proteins by Northern blot analysis. Twelve-week-old male BALB/c mice were operated on to make a closed fracture on the proximal tibiae. On days 2, 4, 7 and 14 after the operation, the fractured bones were excised, fixed with 4% paraformaldehyde (PFA) and decalcified with 20% EDTA to prepare 5-μm sections. Digoxigenin (DIG) labeled single-stranded DNA probes generated by uni-directional polymerase chain reaction (PCR) were used for ISH. Immunohistochemistry revealed fibrin(ogen) at the site of fracture hematoma 2-4 days after fracture and subsequent accumulation of mesenchymal cells. On days 4-14 after the fracture, osteonectin signals were predominantly expressed in proliferating chondrocytes at the endochondral ossification site and in osteoblasts of the marginal woven bone. Osteopontin was expressed on osteoblasts lining the surface of the marginal woven bone at the mid-late phase of fracture healing. <i>In vitro</i>, Northern blot analysis showed that treatment of the mesenchymal cells, C3H10T1/2, with fibrin(ogen) enhanced the steady-state level of osteonectin and osteopontin gene expression. These data suggested that fibrin(ogen) in the hematoma may be important for inducing bone matrix genes in immature mesenchymal cells at the early phase of fracture repair.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 31(6), 501-508, 1998-12-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  20

Cited by:  3

Codes

  • NII Article ID (NAID)
    10008609834
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00445991
  • Data Source
    CJP  CJPref  J-STAGE 
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