無細胞液体培地における鼠らい菌増殖の必須因子はα‐ケトグルタル酸ではなくて培地の至適ｐＨ（６．０～６．２）である

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  • For the Growth of Mycobacterium lepraemurium in Cell-Free Liquid Medium, the Key Essential Factor may be the pH(Optimal 6.0-6.2) of the Culture Medium, rather than the Presence of .ALPHA.-Ketoglutaric Acid.

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Since the success of the multiplication of Mycobacterium lepraemurium in cell-free liquid medium by the author in 1972, various factors affecting its growth have been reported. In particular, it was emphasized that the key essential factor for the growth was α-ketoglutaric acid(α-kg) . However, recent data now indicate that the critical factor for the growth of M.lepraemurium is not α-kg, but the optimal pH of the liquid culture medium, quite similar to the condition of egg-yolk solid medium. The recent experimental results clearly indicated that an abundant multiplication of M.lepraemurium took place in an acid liquid medium containing egg-yolk extract, without α-kg. This finding means that the cells of M.lepraemurium can multiply in a liquid medium, if the pH of the medium is adjusted to 6.0-6.2. The reason why the cells of M.lepraemurium multiplied in NC medium, and NC-5 medium, containing α-kg was that the pH of neutral liquid base medium of the NC or NC-5 medium, was adjusted to 6.0-6.2, optimal for the growth of M.lepraemurium by addition of the 10% α-kg ( where 10% α-kg is strongly acidic ; pH:1.22). Other supplements, such as cytochrome c, 1-cysteine, and hemin, which are routinely added to the base medium of NC and NC-5 medium, have shown stimulatory effects on multiplication of M.lepraemurium, rather than a key essential effect. <BR> In addition, recent experimental data have suggested a second key essential component for growth present in egg-yolk extract.

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