シグナル伝達におけるNAD代謝酵素とサイクリックADPリボース  [in Japanese] NAD^+-cleavage enzymes possibly involved in signal transduction  [in Japanese]

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Author(s)

    • 堅田 利明 KATADA Toshiaki
    • 東京大学薬学部生理化学教室 Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo
    • 紺谷 圏二 KONTANI Kenji
    • 東京大学薬学部生理化学教室 Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo
    • 柊元 巌 KUKIMOTO Iwao
    • 東京大学薬学部生理化学教室 Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo
    • 井上 晋一 INOUE Shin-ichi
    • 東京大学薬学部生理化学教室 Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo
    • 星野 真一 HOSHINO Shin-ichi
    • 東京大学薬学部生理化学教室 Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo
    • 櫨木 修 HAZEKI Osamu
    • 東京大学薬学部生理化学教室 Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo
    • 高橋 勝宣 TAKAHASHI Katsunobu
    • 国立精神・ 神経センター神経研究所疾病研究第三部 Department of Mental Disorder Research, National Institute of Neuroscience

Abstract

The human cell surface antigen CD38 is a 46-kDa type II transmembrane glycoprotein with a short N-terminal cytoplamic domain and a long Cys-rich C-terminal extracellular one. Although CD38 and <I>Aplysia</I> ADP-ribosyl cyclase exhibit amino acid-sequence homology and both catalyze the formation of nicotinamide from β-NAD<SUP>+</SUP>, the other reaction products of the two enzymes are different; ADP-ribose and cyclic ADP-ribose being predominantly generated by CD38 and <I>Aplysia</I> cyclase, respectively. To identify the structural determinant responsible for the different reaction products, we synthesized a series of chimeric proteins between the two enzymes in COS-7 cells. Our results indicated that a limited sequence near the carboxyl terminus of CD38 plays an important role not only in the enzyme activity but also in determination of the enzyme type categorized as a hydrolase. We also investigated the intracellular signaling mediated through CD38 in HL-60 cells which had been caused to differentiate by retinoic acid (RA). Addition of an anti-CD38 monoclonal antibody (mAb) to the cells induced a rapid tyrosine phosphorylation of the cellular proteins with the molecular weights of 120, 000, 87, 000 and 77, 000. Tyrosine kinase activity in the cell lysate immunoprecipitated with anti-phosphotyrosine mAb was also stimulated after the addition of the anti-CD38 mAb. Moreover, one of the prominent phosphorylated proteins stimulated by the anti-CD38 mAb was identified as the <I>cbl</I> proto-oncogene product, p120<SUP>c-cbl</SUP>. These results indicated that tyrosine phosphorylation of cellular proteins including pl20<SUP>c-cbl</SUP> is possibly involved in transmembrane signaling mediated through CD38.

Journal

  • Folia Pharmacologica Japonica

    Folia Pharmacologica Japonica 106, 45-49, 1995-09-01

    The Japanese Pharmacological Society

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