タンニンの活性酸素によるラット腹腔肥満細胞からのヒスタミン遊離に対する抑制効果  [in Japanese] Inhibitory effects of tannins on superoxide-induced histamine release from rat peritoneal mast cells  [in Japanese]

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Author(s)

    • 加納 礼子 KANOH Reiko
    • 徳島文理大学薬学部薬理学教室 Department of Pharmacology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University
    • 波多野 力 HATANO Tsutomu
    • 岡山大学薬学部生薬学教室 Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Okayama University
    • 伊東 秀之 ITOH /Hideyuki
    • 岡山大学薬学部生薬学教室 Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Okayama University
    • 赤木 正明 AKAGI /Masaaki
    • 徳島文理大学薬学部薬理学教室 Department of Pharmacology, Faculty of Pharmaceutical Sciences, Tokushima Bunri University

Abstract

Previously, we demonstrated that potassium superoxide (KO2) elicited degranulation and histamine release from rat peritoneal mast cells by biological reactions. In the present study, we investigated the effects of 20 tannins and 10 antiallergic drugs on KO2 and compound 48/80 (48/80)-induced histamine release from rat peritoneal mast cells. Rat peritoneal mast cells were preincubated with various concentrations of tannins or antiallergic drugs at 37°C for 15 min, and K02 (10 mM) or 48/80 (0.5 μ g/ml) were added. The reactions were continued for 20 min and terminated by chilling the test tubes in an ice bath. The amount of histamine in the supernatant and the residual histamine content were determined fluorometrically. The pretreatment with tannins (1-100 μ M), which have many hexahydroxydiphenoyl (HHDP) groups and galloyl groups, prevented K02-induced histamine release, significantly. Higher molecular weight tannins prevented 48/80-induced histamine release, significantly. The pretreatment with azelastine, astemizole, ketotifen and epinastine prevented K02-induced histamine release, dosedependently. On the other hand, DSCG, repirinast, ibudilast and tazanolast had no effect. These findings indicate that the radical-scavenging activities may participate in the inhibitory effects of tannins, and antiallergic drugs may inhibit histamine release due to membrane-stabilizing action. The inhalation of tannins in the airway may prevent airway hypersensitivity.

Journal

  • Folia Pharmacologica Japonica

    Folia Pharmacologica Japonica 106, 207-211, 1995-09-01

    The Japanese Pharmacological Society

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