生理的状態のラットにおける一酸化窒素の抗血栓性の役割  [in Japanese] Antithrombotic Role of Nitric Oxide in Physiological Rats  [in Japanese]

Access this Article

Search this Article

Author(s)

Abstract

一酸化窒素 (NO) は, 血管拡張因子, 神経伝達物質としての役割を有している一方で, 細胞障害性や血圧低下 (ショック) など, 生体にとって長短両面の作用を有しているが, 生理的状態においてNOが抗血栓性の観点からどの程度の役割を果たしているかは不明である. 今回われわれは, 生理的状態にあるWistar系ラットに対して, NO合成酵素阻害剤であるL-NAMEを投与することにより, NOの果たしている役割を考察した. L-NAME0.8, 4, 20, 100mg/kgの各用量のものを, 4時間かけ尾静脈より点滴静注した. 同剤の投与により, 血中フィブリノゲン, D-dimerは有意に上昇し, TATはL-NAME20mg/kg以上で有意に上昇し, 腎糸球体におけるフィブリン沈着は, 同剤の用量依存性に高度となった. L-NAMEの投与により血栓傾向が惹起されたことは, NOが生理的状態においても生体内における抗血栓作用に重要な役割を果たしていることを示す成績と思われた.

Nitric Oxide (NO) is known to have a variety of functions, and among them are vasodilatation and neurotransmission as favorable functions, and cytotoxicity and hypotension as unfavorable functions. Although NO produced in sepsis has been reported to exhibit an antithrombotic effect, little is known whether or not NO exerts this antithrombotic function under physiological conditions. We have therefore attempted to see whether or not NO manifests any functions against thrombus formation in normal Wistar rats injected with various amounts of L-NAME (N(omega)-nitro-l-arginine methyl ester) an NO synthase inhibitor, i.e. 0.8, 4.0, 20.0 and 100 mg/kg/4hr via the tail vein. Plasma levels of fibrinogen and D-dimer were found to be significantly increased in all rats regardless of the amounts of L-NAME given to the rats, but the thrombin-antithrombin complex was significantly increased in those given 20.0 mg/kg/4hr or more of L-NAME. Fibrin deposition in the glomerulus appeared to become distinct dose-dependently of the drug given to the animals. The finding that administration of L-NAME alone was able to induce the appearance in plasma of the parameters of thrombus formation and fibrin deposition in the glomerulus seems to suggest that NO is involved in the regulation of thrombus formation under physiological circumstances, although its mechanism remains to be clarified.

Journal

  • Japanese Journal of Thrombosis and Hemostasis

    Japanese Journal of Thrombosis and Hemostasis 12(4), 288-293, 2001-08-01

    The Japanese Society on Thrombosis and Hemostasis

References:  31

Cited by:  1

  • Role of Endthelin in LPS-induced RAT DIC Model  [in Japanese]

    SUGA Yukio , ASAKURA Hidesaku , YOSHIDA Tomotaka , MORISHITA Eriko , YAMAZAKI Masshide , AOSHIMA Keiji , ONTACHI Yasuo , MIZUTANI Tomoe , KATO Minori , ITO Takako , MIYAMOTO Ken-ichi , NAKAO Shinji

    Japanese Journal of Thrombosis and Hemostasis 13(2), 169-174, 2002-04-01

    J-STAGE  Ichushi Web  References (27) Cited by (2)

Codes

  • NII Article ID (NAID)
    10008636172
  • NII NACSIS-CAT ID (NCID)
    AN10353762
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    09157441
  • Data Source
    CJP  CJPref  J-STAGE 
Page Top