家兎血漿フィブリノペプタイドAおよびBのHPLCを用いた定量法

• 金城 紀代彦

  琉球大学医学部生理学第一講座

• 中村 真理子

  琉球大学医学部生理学第一講座

• 砂川 昌範

  琉球大学医学部生理学第一講座

• 花城 和彦

  琉球大学医学部生理学第一講座

• 小杉 忠誠

  琉球大学医学部生理学第一講座 The Research Center of Comprehensive Medicine, School of Medicine, University of the Ryukyus

書誌事項

タイトル別名

• Concomitant Measurement of Fibrinopeptide A and B in Rabbit Plasma using High-Performance Liquid Chromatography

抄録

We developed a method for the isolation and estimation of both fibrinopeptide A (FPA) and fibrinopeptide B (FPB) in rabbit plasma using high-performance liquid chromatography with a C 18 column. On analysis of purified fibrinopeptides in phosphate-buffered saline, a good linear relationship between the height count and concentration of peptides (between 1 and 25μg/ml) was observed. On analysis of fibrinopeptides in plasma, which had been prepared with a plasma-precoated C 18 column, FPA and FPB were isolated from the other plasma constituents completely, and a good linear relationship between the height count and concentration of peptides was observed within the range between 3.1 and 12.5μg/ml. 80-100% of the FPA and FPB was recovered within this range. However, at a concentration of 25μg/ml, the recovery values fell to 77% (FPA) and 50% (FPB).

収録刊行物

• 日本血栓止血学会誌

  日本血栓止血学会誌 8 (2), 134-143, 1997

  一般社団法人 日本血栓止血学会

キーワード

• rabbit fibrinopeptides
• plasma-precoated C18 column
• reverse-phase HPLC

詳細情報

• CRID: 1390001204451701504
• NII論文ID: 130004092916
• NII論文ID: 10008638518
• NII書誌ID: AN10353762
• DOI: 10.2491/jjsth.8.134
• ISSN: 18808808
• ISSN: 09157441
• Web Site: https://search.jamas.or.jp/link/ui/1997188307
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可