Characterization of two non-homoeologous nuclear genes encoding mitochondrial alternative oxidase in common wheat

Access this Article

Search this Article

Author(s)

    • Takumi Shigeo TAKUMI Shigeo
    • Faculty of Agriculture, and Division of Life Science, The Graduate School of Science and Technology, Kobe University
    • Tomioka Miki TOMIOKA Miki
    • Faculty of Agriculture, and Division of Life Science, The Graduate School of Science and Technology, Kobe University
    • Eto Kayou [他] ETO Kayou
    • Faculty of Agriculture, and Division of Life Science, The Graduate School of Science and Technology, Kobe University
    • NAYDENOV Nayden
    • Faculty of Agriculture, and Division of Life Science, The Graduate School of Science and Technology, Kobe University
    • NAKAMURA Chiharu
    • Laboratory of Plant Genetics, Department of Biological and Environmental Science

Abstract

Mitochondrial alternative oxidase (AOX) is the terminal oxidase responsible for cyanide-insensitive and salicylhydroxamic acid-sensitive respiration. We have isolated two non-homoeologous genes (<i>Waox1a</i> and <i>Waox1c</i>) encoding AOX proteins from common wheat (<i>Triticum aestivum</i> L.). These two genes were orthologous to rice <i>AOX1a</i> and <i>AOX1c</i>, and their exon/intron structure was conserved, as it is in most other plant <i>AOX</i> genes. Southern blot analysis indicated that both <i>Waox1a</i> and <i>Waox1c</i> were located in at least three homoeologous loci and that additional <i>AOX</i> genes with lower homology were present in the genome of common wheat. The <i>Waox1a</i> and <i>Waox1c</i> loci were respectively assigned to the homoeologous group 2 and 6 chromosomes. The steady-state level of <i>Waox1a</i> and <i>Waox1c</i> transcripts increased under cold stress, while only that of <i>Waox1a</i> was increased by cyanide treatment.<br>

Journal

  • Genes & Genetic Systems

    Genes & Genetic Systems 77(2), 81-88, 2002-04-01

    The Genetics Society of Japan

References:  42

Cited by:  7

Codes

  • NII Article ID (NAID)
    10008639376
  • NII NACSIS-CAT ID (NCID)
    AA11077421
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13417568
  • NDL Article ID
    6264757
  • NDL Call No.
    Z53-W539
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
Page Top