A single-nucleotide mutation in a gene encoding S-adenosylmethionine synthetase is associated with methionine over-accumulation phenotype in Arabidopsis thaliana

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Author(s)

    • Goto Derek B. GOTO Derek B.
    • Laboratory of Molecular Biology, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
    • Ogi Makoto OGI Makoto
    • Laboratory of Molecular Biology, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
    • KUMAGAI Tomoko
    • Laboratory of Molecular Biology, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
    • VAN WERVEN Folkert
    • Laboratory of Molecular Biology, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
    • ONOUCHI Hitoshi
    • Laboratory of Molecular Biology, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University
    • NAITO Satoshi
    • Laboratory of Molecular Biology, Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University

Abstract

Met-overaccumulating mutants provide a powerful genetic tool for examining both the regulation of the Met biosynthetic pathway and <i>in vivo</i> developmental responses of gene expression to altered Met levels. We have previously reported the identification of two <i>Arabidopsis thaliana</i> Met over-accumulation (<i>mto</i>) mutants, <i>mto1-1</i> and <i>mto2-1</i>, that carry mutations in the genes encoding cystathionine γ-synthase (CGS) and threonine synthase (TS), respectively. A third mutant, <i>mto3-1</i>, has recently been reported to carry a mutation in the gene encoding <i>S</i>-adenosylmethionine synthetase 3 (SAMS3). Here, we report the isolation of a new ethionine-resistant <i>A. thaliana</i> mutant that over-accumulates soluble Met approximately 20-fold in young rosettes. The causal mutation was determined to be a single, recessive mutation that was mapped to chromosome 3. Sequence analysis identified a single nucleotide change in the gene encoding SAMS3 that was distinct from the <i>mto3-1</i> mutation and altered the amino acid sequence of the enzyme active site. This mutation was therefore referred to as <i>mto3-2</i>. Although Met over-accumulation in the <i>mto3-2</i> mutant was similar to that in the <i>mto2-1</i> mutant, CGS mRNA levels did not respond to the <i>mto3-2</i> mutation and were similar to that in equivalent wild-type plants.<br>

Journal

  • Genes & Genetic Systems

    Genes & Genetic Systems 77(2), 89-95, 2002-04-01

    The Genetics Society of Japan

References:  29

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008639419
  • NII NACSIS-CAT ID (NCID)
    AA11077421
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13417568
  • NDL Article ID
    6264775
  • NDL Call No.
    Z53-W539
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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