Tr1-copia group retrotransposons in persimmon (Diospyros kaki Thunb.)

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Abstract

We cloned and characterized <i>Ty1-copia</i> group retrotransposons in persimmon (<i>Diospyros kaki</i> Thunb.). Genomic DNA or methyl jasmonate (MJA)-treated cDNA were used as templates to amplify the reverse transcriptase region of <i>Ty1-copia</i> group retrotransposons. About 280 bp fragments were amplified and cloned, and 97 clones were sequenced. Forty-nine clones included frameshift or the stop codon, or both. Among 48 clones containing complete reading frames, 42 clones had unique nucleotide sequences. Alignment and phylogenetic analysis of putative amino acid sequences in the 42 clones indicated that these clones (named <i>Tdk</i>; retroTransposon in <i>Diospyros kaki</i>) fell into seven subgroups and six ungrouped sequences, indicating high sequence heterogeneity in Tdk clones. Phylogenetic analysis comparing unrelated plant species shows that some Tdk clones are more closely related to <i>Ty1-copia</i> group retrotransposons in the orders <i>Solanales</i> and <i>Sapindales</i> rather than to other <i>Tdk</i> clones. Southern blot analysis using <i>Tdk2B, Tdk4c, Tdk6Ac, Tdk12K</i> and <i>Tdk13G</i> clones as probes showed that persimmon and its related species, <i>D. lotus, D. lotus</i> var. <i>glabba, D. oleifera, D. rhombifolia</i> and <i>D. virginiana</i>, contained multiple <i>Tdk</i>-like sequences, indicating that homologous elements exist in other <i>Diospyros</i> species.<br>

Journal

  • Genes & Genetic Systems

    Genes & Genetic Systems 77(2), 131-136, 2002-04-01

    The Genetics Society of Japan

References:  26

Codes

  • NII Article ID (NAID)
    10008639612
  • NII NACSIS-CAT ID (NCID)
    AA11077421
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    13417568
  • NDL Article ID
    6264876
  • NDL Call No.
    Z53-W539
  • Data Source
    CJP  NDL  J-STAGE 
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