環境水のエストロゲン様活性と内分泌攪乱化学物質との関連性  [in Japanese] Contribution of Endocrine-Disrupting Chemicals to Estrogenicity of Environmental Water  [in Japanese]

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Author(s)

    • 中室 克彦 NAKAMURO Katsuhiko
    • 摂南大学 薬学部 環境衛生学研究室 Division of Environmental Health, Faculty of Pharmaceutical Sciences, Setsunan University
    • 上野 仁 UENO Hitoshi
    • 摂南大学 薬学部 環境衛生学研究室 Division of Environmental Health, Faculty of Pharmaceutical Sciences, Setsunan University
    • 坂崎 文俊 SAKAZAKI Fumitosi
    • 摂南大学 薬学部 環境衛生学研究室 Division of Environmental Health, Faculty of Pharmaceutical Sciences, Setsunan University
    • 川井 仁 KAWAI Hitoshi
    • 摂南大学 薬学部 環境衛生学研究室 Division of Environmental Health, Faculty of Pharmaceutical Sciences, Setsunan University
    • 亀井 孝幸 KAMEI Takayuki
    • 摂南大学 薬学部 環境衛生学研究室 Division of Environmental Health, Faculty of Pharmaceutical Sciences, Setsunan University

Abstract

To clarify the contribution of endocrine-disrupting chemicals (EDCs) to the estrogenicity of environmental water, its XAD-2 resin column-concentrates, as well as steroid hormones, alkylphenols and phytoestrogens were assayed by the yeast two-hybrid system with and without S9mix. Lake water, river water, tap water and sewage treatment water exhibited estrogenicity that was enhanced by S9mix. The strong affinity for the estrogen receptor in yeast was observed with steroid hormones containing the 3-position phenolic and 17-position alcoholic hydroxyl groups, and alkylphenols. Among the estrogens, the magnitude of estrogenicity was in the order of: E<sub>2</sub>>E<sub>1</sub>>>E<sub>3</sub>. The estrogenicity of glucuronide or sulfateconjugated estrogens was less than that of free estrogens. However, the estrogenicity of the conjugates of E<sub>1</sub>, E<sub>2</sub> and E<sub>3</sub> was enhanced by S9mix. This suggests that the estrogenicity of environmental water enhanced by the addition of S9mix may be due to the estrogen conjugates in the water. The attenuation of E2's estrogenicity by S9mix was also shown that is predicted to be due to its hydroxylation by P450 induced specifically by phenobarbital. The E<sub>2</sub>-conjugate may be decomposed by some hydrolase expressed primarily in rat liver.

Journal

  • Journal of Japan Society on Water Environment

    Journal of Japan Society on Water Environment 25(6), 355-360, 2002-06-10

    Japan Society on Water Environment

References:  18

Cited by:  6

Codes

  • NII Article ID (NAID)
    10008665807
  • NII NACSIS-CAT ID (NCID)
    AN10372439
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    09168958
  • NDL Article ID
    6183357
  • NDL Source Classification
    ZN5(科学技術--建設工学・建設業--都市工学・衛生工学)
  • NDL Call No.
    Z16-1045
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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