Kinetics for Camel (Camelus dromedarius) Retina Acetylcholinesterase Inhibition by Methotrexate In Vitro

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Abstract

This work addresses the kinetic analysis of the interaction of methotrexate (MTX) with camel retina acetylcholinesterase (AChE, EC 3. 1. 1. 7). It was found that the MTX effect was reversible in nature. The IC<SUB>50</SUB> was determined, by two methods, to be 1.362 mM. The Michaelis-Menten constant (K<SUB>s</SUB>) for the hydrolysis of acetylthiocholine iodide (ASCh) by AChE was 0.123 mM in the control system, and the MTX-treated systems showed a 10-35% decrease in this value. The V<SUB>max</SUB> was 0.789 μmol/min/mg protein for the control system, while it was decreased by 23-76% in the MTX-treated systems. The Lineweaver-Burk plot, Dixon plot and their secondary replots indicated that the inhibition was a linear mixed type; i.e., uncompetitive and noncompetitive. The values of K<SUB>i</SUB> and K<SUB>I</SUB> were estimated as 0.782 and 0.404 mM, respectively. The use of camel retina as a model for the study of human retina may open new avenues for studying various aspects of AChE.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 72(1), 49-55, 1996-09-01

    The Japanese Pharmacological Society

References:  46

Codes

  • NII Article ID (NAID)
    10008675637
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00215198
  • Data Source
    CJP  J-STAGE 
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