Amelioration by CAMP of Cephaloridine-Induced Injury in the Porcine Kidney Cell Line LLC-PK_1

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Author(s)

    • KAWAI Yoshiko
    • Division of Pharmacology, Osaka University of Pharmaceutical Sciences
    • GEMBA Munekazu
    • Division of Pharmacology, Osaka University of Pharmaceutical Sciences

Abstract

We investigated the effects of a phosphodiesterase inhibitor and dibutyryl CAMP (dBcAMP) on the cell injury induced by cephaloridine (CER) in an established renal epithelial cell LLC-PK<SUB>1</SUB>. CER increased the leakage of lactate dehydrogenase (LDH) from LLC-PK<SUB>1</SUB> cells to the medium and the level of lipid peroxidation in the cells. 3-Isobutyl-1-methylxanthine, a phosphodiesterase inhibitor, increased CAMP content in LLC-PK<SUB>1</SUB> cells and ameliorated the increase in LDH Ieakage induced by CER. dBcAMP reduced the cell injury induced by CER. Our results suggest that a signalling pathway of CAMP protects against CER-induced renal cell injury, which is probably due to generation of oxygen radicals.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 72(1), 67-70, 1996-09-01

    The Japanese Pharmacological Society

References:  15

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008675717
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • Data Source
    CJP  CJPref  J-STAGE 
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