Inhibitory Effect of EGTA on Serotonin Transport into Rabbit Blood Platelets: Possible Involvement of the Glycoprotein IIb/IIIa Complex

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We reported previously that serotonin (5-HT) transport was attenuated by treatment of platelets with EGTA, and that this inhibitory effect of EGTA was restored by CaCl<SUP>2•</SUP> In the present study, the inhibitory effect of EGTA was found to be uncompetitive, and no inhibitory effect was observed when EGTA was added at 20°C. Genistein and thyrphostin A47, both protein tyrosine kinase inhibitors, inhibited Ca<SUP>2+</SUP>-induced restoration of 5-HT transport. In contrast, the protein tyrosine phosphatase inhibitor phenylarsine oxide significantly augmented Ca<SUP>2+</SUP>-induced restoration of 5-HT transport. These results might support the hypothesis that the glycoprotein (GP) Ilb/IIIa complex, a platelet membrane integrin protein, might regulate 5-HT transport into blood platelets. It is conceivable that Ca<SUP>2+</SUP> chelation by EGTA might cause temperature-dependent dissociation of the GP IIb/IIIa complex, which results in the reduction of 5-HT transport. Rearrangement of the GP IIb/IIIa complex by replenishment of the Ca<SUP>2+</SUP> binding sites might restore the EGTA-induced reduction of 5-HT transport.

収録刊行物

  • The Japanese journal of pharmacology

    The Japanese journal of pharmacology 72(3), 225-259, 1996-11-01

    The Japanese Pharmacological Society

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各種コード

  • NII論文ID(NAID)
    10008676070
  • NII書誌ID(NCID)
    AA00691188
  • 本文言語コード
    ENG
  • 資料種別
    SHO
  • ISSN
    00215198
  • NDL 記事登録ID
    4080036
  • NDL 雑誌分類
    ZS51(科学技術--薬学)
  • NDL 請求記号
    Z53-D199
  • データ提供元
    CJP書誌  NDL  J-STAGE 
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