Inhibitory Effect of EGTA on Serotonin Transport into Rabbit Blood Platelets:Possible Involvement of the Glycoprotein 2b/3a Complex

• Nishio Hiroaki

  <I>Department of Pharmacology, Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine</I>

• Kagawa Tomomi

  <I>Department of Pharmacology, Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine</I>

• Nezasa Ken-ichi

  <I>Department of Pharmacology, Institute of Pharmaceutical Sciences, Hiroshima University School of Medicine</I>

• Nakata Yoshihiro

書誌事項

タイトル別名

• Inhibitory Effect of EGTA on Serotonin Transport into Rabbit Blood Platelets: Possible Involvement of the Glycoprotein IIb/IIIa Complex.
• Inhibitory Effect of EGTA on Serotonin

抄録

We reported previously that serotonin (5-HT) transport was attenuated by treatment of platelets with EGTA, and that this inhibitory effect of EGTA was restored by CaCl^2• In the present study, the inhibitory effect of EGTA was found to be uncompetitive, and no inhibitory effect was observed when EGTA was added at 20°C. Genistein and thyrphostin A47, both protein tyrosine kinase inhibitors, inhibited Ca²⁺-induced restoration of 5-HT transport. In contrast, the protein tyrosine phosphatase inhibitor phenylarsine oxide significantly augmented Ca²⁺-induced restoration of 5-HT transport. These results might support the hypothesis that the glycoprotein (GP) Ilb/IIIa complex, a platelet membrane integrin protein, might regulate 5-HT transport into blood platelets. It is conceivable that Ca²⁺ chelation by EGTA might cause temperature-dependent dissociation of the GP IIb/IIIa complex, which results in the reduction of 5-HT transport. Rearrangement of the GP IIb/IIIa complex by replenishment of the Ca²⁺ binding sites might restore the EGTA-induced reduction of 5-HT transport.

収録刊行物

• Jpn.J.Pharmacol.

  Jpn.J.Pharmacol. 72 (3), 255-259, 1996

  公益社団法人 日本薬理学会

キーワード

• Serotonin (5-HT) transport
• EGTA
• Glycoprotein IIb/IIIa complex

詳細情報

• CRID: 1390282679263106560
• NII論文ID: 10008676070
• NII書誌ID: AA00691188
• DOI: 10.1254/jjp.72.255
• COI: 1:CAS:528:DyaK28XntFGjsbs%3D
• ISSN: 13473506
• ISSN: 00215198
• NDL書誌ID: 4080036
• PubMed: 8957687
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4080036
• Web Site: https://api.elsevier.com/content/article/PII:S002151981933906X?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S002151981933906X?httpAccept=text/plain
• Web Site: https://search.jamas.or.jp/link/ui/1997083629
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可