Inositol 1,4,5 : Trisphosphate : and Caffeine : Sensitive Ca^<2+> : Storing Organelle in Bovine Adrenal Chromaffin Cells

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Author(s)

Abstract

The uptake and release properties of Ca<SUP>2+</SUP> by several subcellular fractions of the bovine adrenal medulla were investigated. Investigation by the <SUP>45</SUP>Ca<SUP>2+</SUP> tracer method showed that permeabilized cells and the fractions of mitochondria (MT) and microsomes (MC) caused ATP-dependent Ca<SUP>2+</SUP> uptake in a Ca<SUP>2+</SUP> concentration-dependent manner (pCa 8-4), whereas permeabilized cells and the fractions of secretory granules (SG) were able to accumulate a significant amount of Ca<SUP>2+</SUP> even in the absence of ATP, which was completed by the addition of hexokinase and glucose. In these organelle fractions, Ca<SUP>2+</SUP> uptake in the presence of ATP at pCa 7 and pCa 5.8 was well-correlated with the activity of the NADPH cyiochrome c reductase (marker enzyme for the endoplasmic reticulum) and cytochrome c oxidase (marker enzyme for mitochondria), respectively. As detected by Fura-2 ratiometry, both inositol 1, 4, 5-trisphosphate (IP<SUB>3</SUB>) and caffeine caused concentration-dependent Ca<SUP>2+</SUP> releases from permeabilized cells and MC, but not from MT and SG. In an ATP-depleted condition, homogenates still took up a significant amount of Ca<SUP>2+</SUP> but was not able to respond to IP<SUP>3</SUP> and caffeine. These results suggest that the endoplasmic reticulum is a major Ca<SUP>2+</SUP>-storing organelle, which releases Ca<SUP>2+</SUP> in response to IP<SUB>3</SUB> and caffeine in bovine adrenal chromaffin cells.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 72(4), 307-315, 1996-12-01

    The Japanese Pharmacological Society

References:  42

Codes

  • NII Article ID (NAID)
    10008676414
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00215198
  • NDL Article ID
    4121849
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  NDL  J-STAGE 
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