Ouabain : Induced Cell Proliferation in Cultured Rat Astrocytes

Access this Article

Author(s)

Abstract

Ouabain markedly stimulated not only [<SUP>3</SUP>H]thymidine incorporation but also [<SUP>3</SUP>H]uridine incorporation into astrocytes. The effects were observed at 36-48 hr and 12-72 hr after addition of ouabain, respectively. The dose-response curves were both bell-shaped types with a peak at 10<SUP>−3</SUP> M for thymidine incorporation and 2 × 10<SUP>−3</SUP> M for uridine incorporation. Ouabain increased cell number as determined by an assay using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide and by a method using a hemocyiometer. Low concentration of external K<SUP>+</SUP> mimicked the effect of ouabain in stimulating [<SUP>3</SUP>H]-thymidine incorporation, and high concentration of external K<SUP>+</SUP> blocked the effect of ouabain. In contrast to astrocyies, ouabain did not stimulate [<SUP>3</SUP>H]thymidine incorporation into C6 glioma and fibroblast cells. The effect of ouabain on [<SUP>3</SUP>H]thymidine incorporation in astrocyies was dependent on external Ca<SUP>2+</SUP>, and it was blocked by cycloheximide. These findings indicate that prolonged Na<SUP>+</SUP>, K<SUP>+</SUP>-ATPase inhibition causes cell proliferation in cultured astrocyies in cell-specific and Ca<SUP>2+</SUP>-dependent manners.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 72(4), 347-353, 1996-12-01

    The Japanese Pharmacological Society

References:  32

Codes

  • NII Article ID (NAID)
    10008676581
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00215198
  • NDL Article ID
    4121853
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  NDL  J-STAGE 
Page Top