Ouabain-Induced Cell Proliferation in Cultured Rat Astrocytes.

• Murata Yosuke

  <I>Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University</I>

• Matsuda Toshio

  <I>Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University</I>

• Tamada Kazuko

  <I>Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University</I>

• Hosoi Rie

  <I>Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University</I>

• Asano Shoichi

  <I>Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University</I>

• Takuma Kazuhiro

  <I>Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University</I>

• Tanaka Koh-ichi

  <I>Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University</I>

• Baba Akemichi

  <I>Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University</I>

Bibliographic Information

Other Title

• Ouabain Induced Cell Proliferation in C

Abstract

Ouabain markedly stimulated not only [³H]thymidine incorporation but also [³H]uridine incorporation into astrocytes. The effects were observed at 36-48 hr and 12-72 hr after addition of ouabain, respectively. The dose-response curves were both bell-shaped types with a peak at 10⁻³ M for thymidine incorporation and 2 × 10⁻³ M for uridine incorporation. Ouabain increased cell number as determined by an assay using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide and by a method using a hemocyiometer. Low concentration of external K⁺ mimicked the effect of ouabain in stimulating [³H]-thymidine incorporation, and high concentration of external K⁺ blocked the effect of ouabain. In contrast to astrocyies, ouabain did not stimulate [³H]thymidine incorporation into C6 glioma and fibroblast cells. The effect of ouabain on [³H]thymidine incorporation in astrocyies was dependent on external Ca²⁺, and it was blocked by cycloheximide. These findings indicate that prolonged Na⁺, K⁺-ATPase inhibition causes cell proliferation in cultured astrocyies in cell-specific and Ca²⁺-dependent manners.

Journal

• The Japanese Journal of Pharmacology

  The Japanese Journal of Pharmacology 72 (4), 347-353, 1996

  The Japanese Pharmacological Society

Keywords

• Ouabain
• Na<SUP>+</SUP>
• KSUP>+</SUP>-ATPase
• Cell proliferation
• Thymidine incorporation
• Astrocyie

Details

• CRID: 1390001204285057152
• NII Article ID: 10008676581
• NII Book ID: AA00691188
• DOI: 10.1254/jjp.72.347
• COI: 1:CAS:528:DyaK2sXisVantA%3D%3D
• ISSN: 13473506
• ISSN: 00215198
• NDL BIB ID: 4121853
• PubMed: 9015743
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4121853
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819338867?httpAccept=text/xml
• Web Site: https://api.elsevier.com/content/article/PII:S0021519819338867?httpAccept=text/plain
• Web Site: https://search.jamas.or.jp/link/ui/1997210591
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed