Oxidative Stress : Induced Increase in Intracellular Ca^<2+> and Ca^<2+> : Induced Increase in Oxidative Stress: An Experimental Model Using Dissociated Rat Brain Neurons

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Author(s)

Abstract

In order to study the oxidative stress-induced change in intracellular concentration of Ca<SUP>2+</SUP> ([Ca<SUP>2+</SUP>]i) and Ca<SUP>2+</SUP>-induced oxidative stress, effects of hydrogen peroxide and ionomycin, a calcium ionophore, on rat cerebellar neurons were examined using a flow cytometer and fluorescent dyes: fluo-3 for monitoring [Ca<SUP>2+</SUP>]i; 2'', 7''-dichlorofluorescin, for reactive oxygen species; and 5-chloromethylfiuorescein, for cellular nonprotein thiols. Oxidative stress induced by hydrogen peroxide dose-dependently increased [Ca<SUP>2+</SUP>]i and decreased the content of nonprotein thiols. Ionomycin increased oxidative metabolism and decreased the content of nonprotein thiols. Results suggest that oxidative stress induces an increase in [Ca<SUP>2+</SUP>]j While an increase in [Ca<SUP>2+</SUP>]i increases oxidative stress in neurons.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 72(4), 381-385, 1996-12-01

    The Japanese Pharmacological Society

References:  15

Cited by:  3

Codes

  • NII Article ID (NAID)
    10008676670
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    4121857
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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