Dissociation of β-Adrenoceptor Numbers from mRNA Levels during Acute Ischemia in Rat Myocardium

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Author(s)

Abstract

To explore alterations in messenger RNA (mRNA)for the β-adrenoceptor (β3-AR)in ischemic myocardium, we compared the mRNA levels for, β-AR in ischemic and nonischemic myocardium by <I>in situ</I> hybridization using a radioisotope imaging system. We also compared these mRNA levels in ischemic and nonischemic myocardium with the number of the β-AR by radioligand binding assay. The mRNAs for β-AR were diffusely distributed in normal hearts. The level of mRNA detected by <I>in situ</I> hybridization was reduced by acute ischemia, whereas the number of β-AR was increased. Although the number of β-AR was increased in the myocardium with one or three hours ischemia, the total function in β-AR-stimulatory G protein-adenylate cyclase system was not changed. There is a discrepancy between β-AR mRNA and protein levels in the acute ischemic rat ventricular myocardium.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 73(1), 23-28, 1997-01-01

    The Japanese Pharmacological Society

References:  19

Codes

  • NII Article ID (NAID)
    10008676776
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00215198
  • NDL Article ID
    4131791
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  NDL  J-STAGE 
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