Increase of Cl^- Secretion Induced by Kampo Medicine (Japanese Herbal Medicine), Sai-rei-to, in Mongolian Gerbil Middle Ear Epithelium
Access this Article
Search this Article
<I>Sai-rei-to</I>, a type of Kampo medicine (Japanese herbal medicine), has been shown to be clinically effective in treating patients with otitis media with effusion. The effect of <I>Sai-rei-to</I> on the ion transport of the middle ear surface epithelium cultured from the Mongolian gerbil was investigated by using an Ussing chamber. Application of <I>Sai-rei-to</I> to the mucosal bath but not the serosal bath induced an increase in the short-circuit current (I<SUB>sc</SUB>)in the basal state. The increase in I<SUB>sc</SUB> was almost completely inhibited by addition of diphenylamine-2-carboxylic acid but not by amiloride, indicating enhancement of Cl<SUP>-</SUP> secretion. On the basis of the lack of changes in the intracellular Ca<SUP>2+</SUP> concentration and a sideness of action, the effect of <I>Sai-rei-to</I> on I<SUB>sc</SUB> is thought to be a direct and selective activation on the apical Cl<SUP>-</SUP> channel.
- The Japanese Journal of Pharmacology
The Japanese Journal of Pharmacology 73(1), 29-32, 1997-01-01
The Japanese Pharmacological Society