Suppression by Azelastine Hydrochloride of NF : kB Activation Involved in Generation of Cytokines and Nitric Oxide

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Author(s)

Abstract

The influence of the anti-allergy agent azelastine hydrochloride (Azeptin®)on NF-κB activation associated with the. generation of cytokines and nitric oxide (NO)was investigated in various kinds of human and mouse cells. Azeptin dose-dependently suppressed both DNA and protein synthesis in human gingival fibroblasts (HF)and also suppressed blastogenesis of human peripheral blood lymphocytes (PBL). Generation of tumor necrosis factor-alpha, interleukin 1-beta, granulocyte-macrophage colony-stimulating factor and interleukin-6 from 10<SUP>-5</SUP> M Azeptin-treated PBL and human monocytes (HM)was decreased to approximately 1/3 to 2/3 of the control levels. In parallel with the decreased cytokine generation, each cytokine mRNA was less expressed in the presence of 10<SUP>-5</SUP> M Azeptin. In addition, both inducible nitric oxide synthase-mRNA level and NO generation in mouse peritoneal macrophages were suppressed by 10<SUP>-5</SUP> M Azeptin. Being compatible with those results, Azeptin (10<SUP>-5</SUP> M)suppressed activation of NF-κB in PBL, HM and HF. These results appear to indicate that suppression of cytokine and NO generation by Azeptin results at least partially from the inhibition of NF-κB activation.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 73(2), 145-153, 1997-02-01

    The Japanese Pharmacological Society

References:  41

Cited by:  2

Codes

  • NII Article ID (NAID)
    10008677145
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    4153689
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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