Activation of Phorbol Ester Responsive Form of Protein Kinase CC in Association with Ca^<2+> : Induced Differentiation of Primary Cultured Mouse Epidermal Cells

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Author(s)

Abstract

In primary cultured mouse epidermal cells, protein kinase C (PKC) ζconsists of multiple forms: a low salt-eluted PKCζ (1-PKCζ, 79 and 85 kDa) and a high salt-eluted PKCC (h-PKCζ, 79 and 85 kDa) by anion-exchange column chromatography (K. Nishikawa et al., Cell. Signal. 7, 491-504, 1995). In the present study, PKC isozyme-specific responses during terminal differentiation of epidermal cells, which were induced by the increase of Ca<SUP>2+</SUP> -concentration in culture medium, were examined. After 24 hr-treatment with 1.8 mM Ca<SUP>2+</SUP>, 79-kDa 1-PKCζ in the particulate fraction was apparently shifted to the 85-kDa form. The phosphatidylserine-dependent kinase activity of this 1-PKCζ was increased in association with the shift. These results suggest the pivotal role of 1-PKCζ in the particulate fraction in the Ca<SUP>2+</SUP> -induced epidermal cell differentiation processes.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 73(2), 171-174, 1997-02-01

    The Japanese Pharmacological Society

References:  15

Codes

  • NII Article ID (NAID)
    10008677257
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    SHO
  • ISSN
    00215198
  • NDL Article ID
    4153692
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  NDL  J-STAGE 
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