Enhancement of Interleukin-la Mediated Autocrine Growth of Cultured Human Keratinocytes by Sho-saiko-to
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We investigated the effects of <I>Sho-saiko-to</I>, the most commonly used herbal medicine in Japan, on the production of interleukin (IL)-la by cultured human epidermal keratinocytes. IL-lα production was significantly promoted by treatment with 100 or 500 μg/ml Sho-saiko-to for 24 or 48 hr. Expression of IL-lα receptors was the most markedly upregulated after treatment with 500 μg/ml <I>Sho-saiko-to</I> for 24 hr and with 100 or 500 μg/ml for 48 hr; these cells showed the characteristics of multilayered differentiated keratinocytes. The presence of an anti-IL-lα antibody during the treatment with 500 μg/ml of <I>Sho-saiko-to</I> for 24 or 48 hr or with 100 μg/ml for 48 hr significantly down-regulated the synthesis by the keratinocytes and induced damages in them. Keratinocytes treated with <I>Sho-saiko-to</I> might produce IL-lα and express IL-lα receptors. IL-lα may regulate the proliferation and differentiation of keratinocytes after <I>Sho-saiko-to</I> treatment. These findings suggest that <I>Sho-saiko-to</I> enhances the autocrine growth mediated by IL-lα.
- The Japanese Journal of Pharmacology
The Japanese Journal of Pharmacology 73(4), 333-336, 1997-04-01
The Japanese Pharmacological Society