Phorbol 12-Myristate 13-Acetate(PMA)-Induced Oxyradical Production in Rheumatoid Cells

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Author(s)

Abstract

We successfully detected the oxyradical production in human synovial A (macrophage-like)and B (fibroblast-like)cells by phorbol 12-myristate 13-acetate (PMA)using the luminol-chemiluminescence method. The PMA (0.1 μg/ml)-induced photon generation was abolished by an O<SUB>2</SUB><SUP>-</SUP> scavenger, superoxide dismutase, and an H<SUB>2</SUB>0<SUB>2</SUB> scavenger, catalase, suggesting that the stimulus produced oxyradicals in synovial cells. Both of these responses were abolished by a protein kinase C (PKC)inhibitor, calphostine C, but unaffected by an intracellular Ca<SUP>2+</SUP> chelator, BAPTA-AM, and Ca<SUP>2+</SUP> removal from the extracellular medium. These findings suggest that synovial A and B cells produce oxyradicals through PKC-mediated and [Ca<SUP>2+</SUP>]<SUB>i</SUB>-independent mechanisms, probably through the activation of NADPH oxidase.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 73(4), 347-351, 1997-04-01

    The Japanese Pharmacological Society

References:  17

Codes

  • NII Article ID (NAID)
    10008677891
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    SHO
  • ISSN
    00215198
  • NDL Article ID
    4196932
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  NDL  J-STAGE 
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