Phorbol 12-Myristate 13-Acetate (PMA)-Induced Oxyradical Production in Rheumatoid Synovial Cells.

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  • Tanabe Takatoshi
    Department of Pharmacology, Kansai Medical University Department of Orthopaedic Surgery, Kansai Medical University
  • Otani Hitomi
    Department of Pharmacology, Kansai Medical University
  • Mishima Katsuyuki
    Department of Pharmacology, Kansai Medical University
  • Ogawa Ryokei
    Department of Orthopaedic Surgery, Kansai Medical University
  • Inagaki Chiyoko
    Department of Pharmacology, Kansai Medical University

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  • Phorbol 12 Myristate 13 Acetate PMA-Ind

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Abstract

We successfully detected the oxyradical production in human synovial A (macrophage-like)and B (fibroblast-like)cells by phorbol 12-myristate 13-acetate (PMA)using the luminol-chemiluminescence method. The PMA (0.1 μg/ml)-induced photon generation was abolished by an O2- scavenger, superoxide dismutase, and an H202 scavenger, catalase, suggesting that the stimulus produced oxyradicals in synovial cells. Both of these responses were abolished by a protein kinase C (PKC)inhibitor, calphostine C, but unaffected by an intracellular Ca2+ chelator, BAPTA-AM, and Ca2+ removal from the extracellular medium. These findings suggest that synovial A and B cells produce oxyradicals through PKC-mediated and [Ca2+]i-independent mechanisms, probably through the activation of NADPH oxidase.

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