Analysis of Dissociated Single Neurons by Simple and Semi-Quantitative: RT-PCR (Reverse Transcription and Polymerase Chain Reaction)

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Author(s)

Abstract

We have developed a simple and semi-quantitative method for mRNA determination in single cells using the reverse transcription and polymerase chain reaction (RT-PCR). The distinct features of this method are the highly efficient RNA harvest from whole dissociated cells and the ability to perform all RT procedures in one tube that allowed semi-quantitative determination of mRNA in dissociated cells. This method revealed that histamine H<SUB>1</SUB>-receptor mRNA was highly expressed in 5/28 small and 1/26 large dorsal root ganglion neurons of the mouse.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 74(1), 121-123, 1997-05-01

    The Japanese Pharmacological Society

References:  7

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008678639
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    4219994
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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