Analysis of Dissociated Single Neurons by Simple and Semi-Quantitative: RT-PCR (Reverse Transcription and Polymerase Chain Reaction)
We have developed a simple and semi-quantitative method for mRNA determination in single cells using the reverse transcription and polymerase chain reaction (RT-PCR). The distinct features of this method are the highly efficient RNA harvest from whole dissociated cells and the ability to perform all RT procedures in one tube that allowed semi-quantitative determination of mRNA in dissociated cells. This method revealed that histamine H<SUB>1</SUB>-receptor mRNA was highly expressed in 5/28 small and 1/26 large dorsal root ganglion neurons of the mouse.
- The Japanese journal of pharmacology
The Japanese journal of pharmacology 74(1), 121-123, 1997-05-01
The Japanese Pharmacological Society