Flow-Dependent Regulation of Nitric Oxide Formation in the Isolated Canine Mesenteric Arterial Bed

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Abstract

Effects of flow rate changes on nitric oxide (NO)formation in vascular endothelial cells were investigated in isolated canine mesenteric arterial bed preparations. Stepwise increases in the flow rate from 8 ml/min to 40 ml/min significantly (P <0.05)elevated perfusion pressure in a rate-dependent manner. In the presence of N<SUP>G</SUP>-nitro-L-arginine (L-NNA, 100 μM), perfusion pressures were significantly (P<0.01)higher than those observed under control conditions at all flow rates examined. Sodium nitroprusside (SNP)(0.1-10 μM)counteracted the pressor effect of L-NNA in a concentration-dependent manner. Increases in the flow rate from 10 ml/min to 40 ml/min significantly (P < 0.05)augmented cyclic GMP production in the vascular bed preparation. The flow-induced cyclic GMP response was significantly (P<0.05)attenuated by L-NNA (100 μM). These results demonstrate that 1)the amount of NO released from endothelial cells toward vascular smooth muscle cells can be semi-quantified with SNP, and 2)an increase in the flow rate stimulates NO formation in endothelial cells of resistance arteries, which may play an important part in regulating systemic blood pressure.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 74(3), 275-280, 1997-07-01

    The Japanese Pharmacological Society

References:  24

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008679347
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    4260360
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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