Platelet-Derived Growth Factor Blocks the Cell-Cycle Transition from the G_0 to G_1 Phase in Subcultured Angiogenic Endothelial Cells in Rat Thoracic Aorta

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Author(s)

    • Kimura Ikuko KIMURA Ikuko
    • Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University
    • OGASAWARA Masaru
    • Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University

Abstract

Platelet-derived growth factor (PDGF)-BB induces tube formation by the differentiating (tube-forming)endothelial cells (EC)of rat thoracic aorta, although PDGF-BB does not affect the proliferative EC (increasing the cell numbers)at the progression phase. These changes in the responses to PDGF-BB were due to the phenotype-dependent expression of PDGF β-receptor (PDGFR-β)on EC because PDGFR-β-like immunoreactivity was observed in the angiogenic EC forming a tube-like structure in 35-day culture with 10% fetal bovine serum, but not in the proliferative EC in 5-day culture. To elucidate the functional role of PDGFR-β in the alteration of EC phenotype, the influence of PDGF-BB on the cell cycle of EC was investigated by flow cytometry. This analysis demonstrates that PDGF-BB blocks the transition from the G<SUB>0</SUB> to G<SUB>1</SUB> phase in the 35-day cultured EC, although no effect was observed on any phases of the cell cycle in 5-day culture. We conclude that 1)PDGFR-β is expressed in mature angiogenic EC of rat aorta, and 2)PDGF-BB may contribute to promotion of the EC differentiation with tubular morphogenesis by inhibiting cell growth.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 74(4), 303-311, 1997-08-01

    The Japanese Pharmacological Society

References:  18

Cited by:  2

Codes

  • NII Article ID (NAID)
    10008679463
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    4287826
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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